| Check status of cleaved caspase, including cleaved caspase-3 (Asp175), caspase-6 (Asp162), caspase-7 (Asp198), caspase-8 (Asp384) monoclonal, caspase-9 (Asp315) and (Asp330) (human specific). The kit contains enough primary and secondary antibodies to perform two Western mini-blot experiments with each primary antibody. Apoptosis, or programmed cell death, is a regulated physiological process leading to cell death. Caspases, a family of cysteine acid proteases, are central regulators of apoptosis. Initiator caspases (including 8, 9, 10 and 12) are closely coupled to proapoptotic signals. Once activated, these caspases cleave and activate downstream effector caspases (including 3, 6 and 7), which in turn cleave cytoskeletal and nuclear proteins like PARP, a-Fodrin, DFF and Lamin A, and induce apoptosis. Cytochrome c released from mitochondria is coupled to the activation of caspase-9, a key initiator caspase. Proapoptotic stimuli include the FasL, TNF-a, DNA damage and ER stress. Fas and TNFR activate caspases 8 and 10, DNA damage leads to the activation of caspase-9, and ER stress leads to the calciummediated activation of caspase-12. The inhibitor of apoptosis protein (IAP) family includes XIAP and Survivin and functions by binding and inhibiting several caspases (4,5). Smac/Diablo, a mitochondrial protein, is released into the cytosol upon mitochondrial stress and competes with caspases for binding of IAPs. The interaction of Smac/Diablo with IAPs relieves the inhibitory effects of the IAPs on caspases (6). The Cleaved Caspase Antibody Sampler Kit provides an economical means to evaluate the acti-Products Included Product. 1. Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 20ul WB IP IHC IF F H M R Mk 17, 19 Rabbit IgG 2. Cleaved Caspase-6 (Asp162) Antibody 20ul WB H M R 18 Rabbit 3. Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb 20ul WB IF F H M R 18 Rabbit IgG 4. Cleaved Caspase-9 (Asp330) (E5Z7N) Rabbit mAb 20ul WB IF H 37 Rabbit IgG 5. Cleaved Caspase-9 (Asp315) Antibody (Human Specific) 20ul WB IP H 35 Rabbit 6. Cleaved PARP (Asp214) (D64E10) Rabbit mAb 20ul WB IP IHC IF F H Mk 89 Rabbit IgG 7. Anti-rabbit IgG, HRP-linked Antibody 100ul WB Goat |
