Catalase is a hemoprotein containing four subunits which decomposes peroxide. Catalytic activity is present in nearly all animal cells and organs and in aerobic microorganisms. Activity: 20,000 units/mg protein Unit Definition: 1 unit decomposes 1umole of hydrogen peroxide/minute at 25C, pH 7.0. Composition: 4 subunits of equal size (MW 60,000-65,000) Quality Control: SDS-PAGE Handling: Contains thymol as a preservative. To remove thymol, measure desired amount of mixed suspension, centrifuge to collect enzyme crystals, remove supernatant. Resuspend crystals in one-half initial volume of water, respin. Discard wash supernatant and dissolve crystals in buffer of choice. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Protect from moisture.
