Purified Human Catalase from red cells (erythrocytes)
Highly reactive and potentially dangerous reactive oxygen species (ROS) are normally produced within the cells, primarily from the mitochondrial respiratory chain where in excess electrons are donated to molecular oxygen (O2) to generate peroxide anion (O2-). Superoxide anion is reduced by the superoxide dismutase (SOD) to hydrogen peroxide (H2O2) and hydrogen peroxide is reduced to water (H2O) by catalase, located primarily in the peroxisomes, and by glutathione peroxidase (GPx), located in the mitochondria and cytosol. Hydrogen peroxide, in the presence of transitions metals, can be converted to the highly toxic hydroxyl radical (OH.) and all three of the ROS (O2-, H2O2, and OH.) can damage macromolecules (proteins, DNA etc). The GPxs are commonly considered the most important for ROS defense since they have broader substrate specificities and stronger affinity for H2O2 than catalases. Catalase (CATA, 527 aa, chromosome 11p13) is peroxisomal enzyme found in almost all aerobically respiring organisms and serves to protect cells from the toxic effects of hydrogen peroxide. It is homotetramer. Defects in CAT are the cause of acatalasia or acatalasemia. This disease is characterized by absence of catalase activity in red cells and is often associated with ulcerating oral lesions. Applications: Western Blot: 1:500-1:2000 using ECL technique. A 60-65kD band (under reducing condition) is observed. Native protein is ~256kD. ELISA Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by salt fractionation and DEAE column chromatography.
Form:
Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
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