Synthetic peptide corresponding to residues around Ser37 of human beta-catenin. Species sequence homology: porcine and zebrafish
Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1). It has been implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3). GSK-3 beta or other kinases may destabilize beta-catenin by phosphorylating it at serines 33, 37, 45 and threonine 41 (4). Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (5). Applications: Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1:1000 Immunoprecipitation: 1:100 Immunohistochemistry (Paraffin): 1:400 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
