Synthetic phosphopeptide corresponding to residues surrounding Thr41 and Ser45 of human beta-catenin. Species sequence homology: canine and porcine
Beta-catenin is one of the key downstream effectors in the Wnt signaling pathway (1). It has been implicated in two major biological processes of vertebrates: early embryonic development (2) and tumorigenesis (3). GSK-3beta or other kinases may destabilize beta-catenin by phosphorylating it at serines 33, 37, 45 and/or threonine 41 (4). Stabilizing mutations of these phosphorylation sites in beta-catenin have been found in many tumor cell lines (5). Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
