MaxLight(TM) 490 is a new Blue-Green photostable dye conjugate comparable to DyLight(TM)488, Alexa Fluor(TM)488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm), Emission (515nm), Extinction Coefficient 73,000. Cathepsin B is a cysteine protease commonly overexpressed in metastisizing cancer cells. An intracellular (lysosomal) enzyme in peptidase family C1 (papain family), Cathepsin B will hydrolyze proteins with broad specificity for peptide bonds. Preferentially cleaves -Arg-Arg bonds in small molecule substrates (thus differing from cathepsin L). In addition to being an endopeptidase, shows peptidyl-dipeptidase activity, liberating C-terminal dipeptides. Applications: Suitable for use in FLISA and Western Blot. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
Purity:
Purified
Form:
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight(TM)490.
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