CD27, Mouse IgG2a Fc Fusion Protein (CD27 Antigen, CD27 Molecule, CD27L Receptor, MGC20393, S152, T-cell Activation Antigen CD27, T14, Tp55, Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7) (Biotin)

Product Overview

• Article Name: CD27, Mouse IgG2a Fc Fusion Protein (CD27 Antigen, CD27 Molecule, CD27L Receptor, MGC20393, S152, T-cell Activation Antigen CD27, T14, Tp55, Tumor Necrosis Factor Receptor Superfamily Member 7, TNFRSF7) (Biotin)
• Biozol Catalog Number: USB-C2379-12F
• Supplier Catalog Number: C2379-12F
• Alternative Catalog Number: USB-C2379-12F-25
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: ELISA, FC

Product Description

Human CD27 is a lymphocyte specific member of the tumor necrosis factor receptor family (TNFRSF7) and is found primarily on peripheral blood T cells and on a subpopulation of B cells and NK cells. The ligand for CD27 is CD70, which is a member of the TNF ligand superfamily (TNFSF7). The CD27-CD70 interaction plays an important role in T cell activation. Reactivity: Recombinant soluble CD27-muIg binds to cell surface CD70 on Raji cells in FACS, and is reactive with recombinant CD70-muCD8 and CD27. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 cultured human Raji cells were washed and pre incubated 5 minutes with 20ul of 300ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80ul of C2379-12F at 10ug/ml. 2. Cells were washed twice and incubated with secondary reagent Streptavidin/R-PE (Cat S7973-88P), after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 0.56 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. 4. Binding was blocked when reagent was pre incubated with a 5-fold excess (mg/mg) of recombinant soluble CD70-muCD8 (Cat C2425-01Q). Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Purity: Purified by Protein A affinity chromatography.
• Form: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with Biotin.