Human CD28 is an important costimulatory molecule found on all CD4+ T cells and on about half of the CD8+ T cells. T cell activities attributed to CD28 include, prevention of anergy, induction of cytokine gene transcription, stabilization of cytokine mRNAs and activation of CD8+ cytotoxic T lymphocytes. The ligands for CD28, identified as CD80 (B7-1) and CD86 (B7-2), are immunoglobulin superfamily monomeric transmembrane glycoproteins of 60kD and 80kD, respectively. Molecular Structure: A soluble fusion protein consisting of the extracellular (134 aa) domain of human CD28 fused to murine IgG2a Fc (232 aa), with a predicted monomeric molecular weight of 41.8kD. Transfectant Cell Line: CHO Human CD28-muIg/Biotin Fusion Protein Functional Application: CD28-muIg fusion protein blocks binding of anti-human CD28 to human tumor cells. Applications: Suitable for use in ELISA and Antibody Blocking. Other applications not tested. Recommended Dilution ELISA: 0.5ng/ml detects the fusion protein using an anti-CD28 antibody coated capture plate and Streptavidin/HRP as a detector. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
41.8
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 5% glycerol, 0.04% sodium azide. Labeled with Biotin.
* VAT and and shipping costs not included. Errors and price changes excepted
