CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4) (Biotin), IgG

Product Overview

• Article Name: CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4) (Biotin), IgG
• Biozol Catalog Number: USB-C2404-46K-BIOTIN
• Supplier Catalog Number: C2404-46K-Biotin
• Alternative Catalog Number: USB-C2404-46K-BIOTIN-100
• Manufacturer: US Biological
• Host: Mouse
• Category: Antikörper
• Application: FC, IHC, IP
• Immunogen: JM leukemia line.

Product Description

CD49d is a ~150kD single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kD (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyers patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997). CD49d can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure and has been demonstrated to inhibit cell binding to soluble VCAM-1 (Weller et al. 1991). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug/10e5 cells Immunohistochemistry: Frozen Functional Assays: Removal of sodium azide by dialysis is recommended prior to use. Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [10B1722 HP2/1]
• Isotype: IgG1
• Purity: Purified by Protein G affinity chromatography.
• Form: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.