CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4) (MaxLight 650

Product Overview

• Article Name: CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4) (MaxLight 650
• Biozol Catalog Number: USB-C2404-46K-ML650
• Supplier Catalog Number: C2404-46K-ML650
• Alternative Catalog Number: USB-C2404-46K-ML650-100
• Manufacturer: US Biological
• Host: Mouse
• Category: Antikörper
• Application: FC, IHC, IP
• Immunogen: JM leukemia line.

Product Description

MaxLight(TM)650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor(TM)647, DyLight(TM)649, Cy5(TM) and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm), Emission (676nm), Extinction Coefficient 250,000. CD49d is a ~150kD single pass type 1 transmembrane glycoprotein with seven FG-GAP repeats, characteristic of alpha integrins, in its extracellular domain. CD49d can be proteolytically cleaved to yield framents of 80 and 70kD (Hemler et al. 1987). CD49d associates with either CD29 to form VLA-4 or with Integrin beta-7 to form The Peyer patches-specific homing receptor LPAM-1, involved in the lymphocyte migration and homing to gut-associated lymphoid tissue (Sackstein 2006) through its interaction with MadCam-1, preferentially expressed on Peyers patch high endothelial venules and postcapillary venules in lamina propria (Briskin et al. 1997). CD49d can be used in basic studies of VLA-4 mediated adhesion and its interaction with the VCAM-1 structure and has been demonstrated to inhibit cell binding to soluble VCAM-1 (Weller et al. 1991). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: 1ug/10e5 cells Immunohistochemistry: Frozen Functional Assays: Removal of sodium azide by dialysis is recommended prior to use. Optimal dilutions to be determined by the researcher. Hybridoma: X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Product Properties

• Clonality: Monoclonal
• Clone Designation: [10B1722 HP2/1]
• Isotype: IgG1
• Purity: Purified by Protein G affinity chromatography.
• Form: Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight(TM)650.