Applications: Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation, Flow Cytometry and ELISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry: For use on acetone or paraformaldehyde fixed tissue Immunocytochemistry: Will react with some lymphoid cell lines (B cells), many carcinoma and melanoma cell lines and osteosarcomas. Optimal dilution determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.