Human CD53 is a tetraspan cell surface glycoprotein found on all hemopoetic cells except platelets and red blood cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 5ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultures Jurkat cells/tube were incubated 45 minutes on ice with 80ul of C2408-11H at 5ug/ml. 2. Cells were washed twice and incubated with IgG (FITC) goat anti-mouse, after which they were washed three times, fixed and analyzed by FACS. 3. Cells stained positive with a mean shift of 1.03 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[63.5A3]
Isotype:
IgG2b
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant.. >95% by SDS-PAGE.
Form:
Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride. Preservative Free
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