CD54 (D1) (Intercellular Adhesion Molecule 1, ICAM-1, Major Group Rhinovirus Receptor, ICAM1), Clone: [15.2], Mouse, Monoclonal
Biozol Catalog Number:
USB-C2409-11M
Supplier Catalog Number:
C2409-11M
Alternative Catalog Number:
USB-C2409-11M-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
ELISA, FC, IHC, WB
Immunogen:
Human monocytes
Human CD54 (ICAM-1) mediates cell adhesion by binding to the integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). CD54 expression on resting peripheral blood leukocytes is weak but is upregulated on activated T and B lymphocytes and on monocytes. Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: Capture Immunohistochemistry: Fixed cells and tissue Flow Cytometry: 1ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x10e5 cultured Raji cells were incubated 45 min on ice with 80ul of C2409-11M at 1ug/ml. 2. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. 3.Cells stained positive with a mean shift of 1.58 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[15.2]
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
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