Human CD59 is a GPI anchored molecule that protects cells from complement-mediated lysis (1). Studies are progressing to determine the regulatory mechanism for CD59 expression (2). Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml labels 5x10e5 cultured human HPB-MLT cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 cultured HPB-MLT human tumor cells were washed and preincubated 5 minutes with 20ul of 250ug/ml human IgG (to block nonspecific binding). 2. After which they were incubated 45 minutes on ice with 80ul of C2413-21P at 10ug/ml. 3. Cells were washed twice and incubated with S7973-88P: Streptavidin (PE), after which they were washed three times, fixed and analyzed using FACS. 4. Cells stained positive with a mean shift of 1.9 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20ul of 0.5mg/ml of 0.5 mg/ml anti-CD59 antibody. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[7H165]
Purity:
Purified by Protein A affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.5, 0.2% BSA, 0.04% sodium azide, 5% glycerol.
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