CD59 (16.3A5, 1F5, CD59a, EJ16, EJ30, EL32, FLJ38134, FLJ92039, G344, 20kD Homologous Restriction Factor, HRF20, HRF-20, Human Leukocyte Antigen MIC11, Ly-6-like Protein, Lymphocytic Antigen CD59/MEM43, MAC Inhibitory Protein, MAC
Biozol Catalog Number:
USB-C2413-21V
Supplier Catalog Number:
C2413-21V
Alternative Catalog Number:
USB-C2413-21V-200
Manufacturer:
US Biological
Host:
Rat
Category:
Antikörper
Application:
FC, IHC, WB
Immunogen:
Human Peripheral Blood T cells.
CD59 cell surface antigen is also known as MAC-inhibitory protein, 1F5 antigen, 20 kDa homologous restriction factor, MEM43 antigen, membrane attack complex inhibition factor, membrane inhibitor of reactive lysis or protectin. CD59 is produced as a 103 amino acid proprotein, further processed to produce an 18-20 kDa GPI linked glycoprotein broadly expressed by human leucocytes and erythrocytes. Mutation of the CD59 gene can result in the presentation of Hemolytic anemia, CD59-mediated, with or without polyneuropathy (HACD59) a disease characterized by infantile onset of a relapsing-remitting polyneuropathy manifest as limb muscle weakness, and hyporeflexia. C2413-21V, clone YTH53.1 blocks the normal function of CD59 (Walsh et al. 1991). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry (frozen and paraffin), and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100, 10ul labels 10e6 cells in 100ul Immunohistochemistry (paraffin): 1:5-1:10 Optimal dilutions to be determined by the researcher. Hybridoma: Y3/Ag1.2.3 rat myeloma cells with spleen cells from DA rat. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
