MaxLight(TM)490 is a new Blue-Green photostable dye conjugate comparable to DyLight(TM)488, Alexa Fluor(TM)488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm), Emission (515nm), Extinction Coefficient 73,000. Recommended Secondary Reagents: F(ab)2 rabbit anti-mouse IgG HRP, F(ab)2 rabbit anti mouse IgG: RPE conjugate Recommended Negative Controls: Mouse IgG2a Negative Control Applications: Flow Cytometry: 1:20 Immunohistochemistry: (frozen)- 0.5-2ug/ml Optimal working dilutions to be determined by researcher. Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul. Note: Applications are based on unconjugated antibody.
Clonality:
Monoclonal
Clone Designation:
[3H1606]
Isotype:
IgG2a
Purity:
Purified IgG.
Form:
Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight(TM)490.
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