CD62E represents the 97kD type I transmembrane glycoprotein E-selectin, also known as endothelial-leukocyte adhesion molecule-1 (ELAM-1). It is expressed on cytokine-stimulated endothelium, weakly on unactivated endothelium, and is absent on leukocytes. Like other selectins, CD62E is an adhesion molecule that contributes to the initial attachment and rolling of leukocytes on endothelial surfaces, a prerequisite for leukocyte extravasation into tissues. CD62P is a 140kD membrane glycoprotein formerly known as platelet activation-dependent granule membrane protein (PADGEM) or GMP-140. It is expressed on platelets, megakaryocytes, and epithelium. It is thought to mediate the adhesive interactions of neutrophils and monocytes with endothelium in inflammatory responses and of activated platelets to neutrophils and monocytes in hemostasis. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot, ELISA and Inhibition of activity. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
