CD62L is expressed on most peripheral blood T cells, B cells, some NK cells, monocytes and neutrophils. CD62L is also expressed on some splenic lymphocytes, bone marrow lymphocytes and myeloid cells, thymocytes and certain hematopoietic malignant cells. CD62L acts as the peripheral lymph node homing receptor, mediates lymphocytes homing to high endothelial venules (HEV) of peripheral lymphoid tissue and leukocytes rolling on activated endothelium at inflammatory sites. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Immunohistochemistry (Paraffin): 10ug/ml Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul. Optimal dilutions to be determined by the researcher. Recommended Immunohistochemistry Positive Control Tissue: Human Tonsil. Hybridoma: NS1 myeloma cells with spleen cells from Balb/c mice. Recommended Secondary Antibodies: I1904-23: IgG, Mouse, Cross Adsorbed (FITC) I1904-09L: IgG, H&L, X-adsorbed (FITC) Pab Gt xMo Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
