CD62L is a 74-95 kD glycoprotein,also known as L-selectin, LECAM-1, Ly-22 and MEL-14. It is a member of the selectin family and is expressed on the majority of B and naive T cells, a subset of memory T cells,granulocytes, subsets of thymocytes and NK cells. CD62L is important in lymphocyte homing to high endothelial venules in peripheral lymph nodes and leukocyte ''rolling on activated endothelium. CD62L also contributes to neutrophil emigration at inflammatory sites. CD62L is rapidly shed from lymphocytes and neutrophils upon activation and the expression levels of CD62L (in conjunction with other markers) have been used to distinguish naive, effector and memory T cells. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: 1ug labels 10e6 cells in 100ul. Optimal working dilutions to be determined by researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Clonality:
Monoclonal
Clone Designation:
[3H1613 OX-85]
Purity:
Purified by affinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
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