CD63 is a lysosomal granule-membrane glycoprotein and has been detected on the surface and in the cytoplasma of a variety of cells, particularly in platelets, surface expression indicates lysosomal secretion. CD63, present only in azurophil granules of non-stimulated neutrophils, is strongly expressed at the surface of neutrophils after activation showing that CD63 is a good marker for granule release, this phenomenon has been also reported for basophils. CD63 is present in cell line PHA blasts, K562, U937, KG1a, RAJI. Both surface and cytoplasmic reactions are reported. CD63 antigen has a diverse distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunofluorescence. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:25-1:100. Use 10ul to label 10e6 cells in 100ul or 100ul whole blood. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
