hCD70-muCD8 Fusion Protein (human CD70-murine CD8 Fusion Protein)
Biozol Catalog Number:
USB-C2425-01QX
Supplier Catalog Number:
C2425-01QX
Alternative Catalog Number:
USB-C2425-01QX-25
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA, FC
Human CD70 (TNFSF7) is the ligand for CD27 (TNFRSF7) and is notably absent on resting lymphocytes. CD70 is expressed after activation on about 20% of T cells and on about 70% of B cells. Recombinant CD70-muCD8 binds to cell surface CD27 on human peripheral blood leukocytes. Molecular Structure: A soluble molecule consisting of the extracellular domain (167aa) of murine CD8 alpha fused to 146 (c-term) amino acids of the extracellular domain of human CD70, with a predicted monomeric weight of 34.8kD. Extracellular mature murine CD8alpha: (28)KPQAPEL....GLDFACD(194), Linking amino acids: GT, extracellular human CD70: (48)LPLESL....VQWVRP(193). Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ug/ml, 80ul labels 5x10e5 ficoll prepared human peripheral blood mononuclear cells. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre incubated for 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding). 2. They were incubated for 45 minutes on ice with 80ul of C2425-01QX at 10ug/ml. 3. Cells were washed twice and incubated with C2259-11D, after which they were washed three times, fixed and analyzed by FACS. 4. A net 57% sub population of the cells stained positive with a mean shift of 1.15 log10 fluorescent units when compared to a buffer control. Binding was 99% blocked when reagent was first pre incubated with an excess of CD-70 antibody. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Molecular Weight:
34.8
Purity:
Purified by affinity chromatography.
Form:
Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
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