CD112 Poliovirus Receptor Related 2 (PRR2), is a new surface molecule, which has been reported to be homologous to the poliovirus receptor (CD155). Studies demonstrate that PRR2 expression is restrictred to the myelomonocytic and megakaryocytic lineages. PRR2 expresion has also been detected on haematopoeitic progenitors expressing CD34. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Not recommended for use in Western Blot. Other applications have not been tested. Recommended Dilutions: Immunohistochemistry: Frozen sections Optimal working dilutions to be determined by researcher. Recommended Secondary Antibody: I1904-14C: IgG, Fc (HRP) Pab Gt xMo I1904-23: IgG, X-Adsorbed (FITC) Pab Gt xMo Recommended Negative Control: I1904-78R: IgG1 Murine Negative Control Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
