Human CD134L (OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L binds to CD134 present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of immflamation and rejection (5,6,7,8). Functional Application: CD134L-muCD8 fusion protein binds to CD134 positive cells in Flow cytometry. Human CD134L-muCD8 Fusion Protein* Molecular Structure: A soluble molecule consisting of the extracellular domain (132 aa) of human CD134L fused to the extracellular domain (167aa) of murine CD8 alpha. Transfectant Cell Line: CHO Applications: Suitable for use in ELISA, Flow cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: protein binds to CD134 positive cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
34.1
Purity:
Purified by affinity chromatography.
Form:
Supplied as a liquid in 50mM sodium phosphate, 100mM Potassium chloride, 150mM sodium chloride, pH 7.5, 0.5 mg/ml Gentamicin Sulfate.
* VAT and and shipping costs not included. Errors and price changes excepted
