Human CD137 Ligand is a type II transmembrane protein constitutively expressed by monocytes, B cells and neuroblastoma cells (1). Binding of CD137 (4-1BB) to CD137L induces monocyte activation (2). Human CD137L-muCD8 binds to CD137 on human cells and is blocked by CD137-huIg fusion protein. Functional Application: Human CD137L-muCD8 binds to CD137 and blocks binding of anti-CD137 monoclonal antibody. Human CD137L-muCD8/Biotin Fusion Protein* Molecular Structure: A soluble fusion protein consisting of the extracellular domain (184aa) of human CD137 Ligand fused to the extracellular domain (167aa) of murine CD8 alpha. Transfectant Cell Line: CHO Applications: Suitable for use in ELISA, Flow cytometry. Other applications not tested. Recommended Dilution Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
38.2
Purity:
Purified by affinity chromatography.
Form:
Supplied as a liquid in FBS, 50mM Sodium Phosphate pH 7.5, 100mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3.
* VAT and and shipping costs not included. Errors and price changes excepted
