Immune response mediated by T cells can be characterized to functionally proceed as follows: antigen recognition by the T cell receptor, activation through costimulation, effector activities to eliminate antigen and finally down regulation. Human CD152 is a cell surface glycoprotein expressed at low levels on activated T cells. CD152 is a high affinity receptor for the costimulatory molecules CD80 (B7-1) and CD86 (B7-2) and appears to function as a negative regulator of T cell activation. Therefore, CD152 may be an important player in down regulating T cell mediated immune responses Molecular Structure: A soluble 110kD dimeric fusion protein consisting of the extracellular (125aa) domain of human CTLA-4 fused to murine IgG2a Fc (233aa), with a predicted non glycosylated monomeric molecular weight of 40.1kD. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Functional Application: Binds with high affinity to human or mouse CD80 (B7-1) and CD86 (B7-2) (4, 5). Blocks the binding of anti-human CD80 (B7-1) and anti-human CD86 (B7-2) mAbs to recepters on Raji cells. Crossreacts with CD80 and CD86 across a broad range of mammalian species including pig (6). Recommended Dilutions: Flow Cytometry:5ug/ml. 80ul of diluted C2548-83VX labels 5x10e5 cultured Raji cells. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
40.1
Purity:
Purified by Protein A affinity chromatography from tissue culture supernatant. (>95% by SDS-PAGE with <1% bovine immunoglobulin)
Form:
Supplied as a sterile-filtered liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
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