CDw124, Recombinant, Human (Interleukin 4 Receptor alpha, Soluble, IL-4Ra)
Biozol Catalog Number:
USB-C2587-60A
Supplier Catalog Number:
C2587-60A
Alternative Catalog Number:
USB-C2587-60A-3,USB-C2587-60A-15
Manufacturer:
US Biological
Category:
Molekularbiologie
Human IL-4 exerts its biological effects via signaling through its receptor system, IL-4R. There are two types of the IL-4 receptor complexes, the type I receptor consisting of the IL-4R alpha and the common gamma chain, yc and the type II receptor consisting of IL-4R alpha and the IL-13R alpha. The IL-4R alpha chain contains an extracellular domain of 207 amino acids, a transmembrane domain of 24 amino acids, and an intracellular domain of 569 amino acids. The secreted extracellular domain, sIL-4R alpha, also called CD124 is capable of blocking IL-4 activities. This receptor plays a major role in the regulation of the differentiation of naive CD4+ T cells and class switching to IgG1 and IgE. Recombinant human sIL-4Ralpha is a 209 amino acid protein which corresponds to the entire extracellular domain of IL-4Ralpha. Amino Acid Sequence: GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT GNMKVLQEPT CVSDYMSIST CEWKMNGPTN CSTELRLLYQ LVFLLSEAHT CIPENNGGAG CVCHLLMDDV VSADNYTLDL WAGQQLLWKG SFKPSEHVKP RAPGNLTVHT NVSDTLLLTW SNPYPPDNYL YNHLTYAVNI WSENDPADFR IYNVTYLEPS LRIAASTLKS GISYRARVRA WAQCYNTTWS EWSPSTKWHN SYREPFEQH Biological Activity: The ED50 was determined by its ability to inhibit the IL-4 dependent proliferation of human TF-1 cells is 5ng/ml (in the presence of 0.5ng/ml of IL-4), corresponding to a specific activity of 2x10e5 units/mg. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
