Recombinant Human C-JUN amino acids 1-81 produced in E.coli is a non-glycosylated, polypeptide chain having a molecular mass of 52kD. Recombinant C-JUN is a maltose binding protein (MBP) fusion protein with an amino-terminal polyhistidine tag and purified by proprietary chromatographic techniques. Biological Activity: Recombinant human c-Jun is phosphorylatable in vitro, using either recombinant active JNK1 or JNK2, or with JNK immunoprecipitated from stimulated cells. This phosphorylation can be monitored by Western blot analysis using an antibody directed to c-Jun [pS73], in conjunction with chemiluminescence detection methods. Optimization of the cell stimulation protocol, cell lysis procedure, and reaction conditions may be required for each specific application. Please note: Kinase activity may vary depending on the substrate and reaction conditions. Storage: Store at 4C if entire vial will be used within 1-2 weeks. Store, frozen at -20C for longer periods of time. Please avoid freeze-thaw cycles.
