FITC-labeled bovine type I is an excellent substrate for examining collagenase activity. This labeled substrate is highly purified and telo-peptide free. To minimize background levels in collagenase assays, FITC-labeled collagen has been enzymatically pre-treated and further purified by ion-exchange chromatography. Applications: Suitable for use in the assay of mammalian collagenase activity. Solubility: FITC-bovine type I collagen is soluble in acidic buffer (maximum 4mg/ml), but difficult to dissolve in neutral buffer. In order to dissolve in neutral buffer, dissolve in 0.01M acetic acid at 4mg/ml, and then add into neutral buffer (final NaCl concentration should be 0.15-0.2M). FITC-bovine type I collagen forms stable gel in neutral buffer and can be used as a substrate for assaying collagenase activity by collagen-gel method. Storage and Stability: Store at -20C in the dark. The collagen solution is stable in acidic solution at -20C but will gradually degrade under neutral conditions. Collagen will form insoluble gel and degrade under neutral conditions during long-term storage at 4C.
Molecular Weight:
300
Purity:
99% by SDS-PAGE. Background value is less than 0.1% (free fluorescence intensity (FI) extracted with 35% ethanol at 25C).
Form:
Suppllied as liquid in 0.01M acetic acid. Labeled with FITC. Molar ratio: 1:1
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