Type I collagen is the major structural component of extracellular matrices found in connective tissue and internal organs, but is most prevalent in the dermis, tendons, and bone. It is a 300kD molecule composed of two alpha 1(I) chains and one alpha2(I) chain that spontaneously forms a triple helix scaffold when at a neutral pH and 37C. This phenomenon can be exploited to promote cell attachment, growth, differentiation, migration, and tissue morphogenesis during development. Quality Control: Tested for uniform gelation and for attachment of and spreading of HT-1080 human fibrosarcoma cells. Negative for the presence of mycoplasma, bacteria and fungi. Technical Notes: 1. Typically if the rat tail collagens concentration goes below 0.4mg/ml, there will be little to no gelation. 2. Higher pH can also interfere with the gelling of this product. pH: 3.4-3.6 Endotoxin: 20EU/ml (LAL) Storage and Stability: May be stored at 4C. For long-term storage, aliquot and store at 4C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Quality Control: Tested for uniform gelation and for attachment of and spreading of HT-1080 human fibrosarcoma cells. Negative for the presence of mycoplasma, bacteria and fungi. Endotoxin: 20EU/ml by LAL assay
Form:
Supplied as a liquid in 20mM acetic acid.
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