Applications: Type II collagen standard Antigen for antibody production Coating material for cell culture studies Formation of collagen gels Reconstitution: The collagen may be reconstituted by adding 500mM Acetic Acid. The collagen may be transferred into physiological buffers by dialysis at 2-8C. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile 500mM Acetic Acid. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Controlled and limited pepsin digestion, followed by selective salt precipitation. > 90% as judged by SDS-PAGE analysis.
Form:
Supplied as a lyophilized solid.
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