Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600A in length and 15A in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Highly purified type II collagen. Used for the induction of collagen-induced arthritis (CIA) in experimental animals. Suitable for use as an antigen in immunological protocols. Applications: Suitable for use as a standard for collagen analysis by SDS-gel, substrate for MMP-1, MMP-8, and MMP-13, and as an antigen for immunizing animals for the induction of collagen-induced arthritis (CIA) in certain species and strains of experimental animals. Other applications not tested. Recommended Dilutions: Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20C. Reconstitute with sterile acidic acid. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Molecular Weight:
300
Purity:
99% by SDS-PAGE, free of pepsin and proteoglycans.
Form:
Supplied as a lyophilized powder. Reconstitute with 0.01M or 0.05M acidic acid at 1-4mg/ml. Futher dilution can be made in neutral buffer. acidic buffer to 4mg/ml. Does not dissolve in neutral buffer.
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