Collagen is an inert, rigid protein found predominantly in skin, ligaments, bones and teeth. Its most distinctive attribute, essential to a transmitter of mechanical force, is inelasticity. Its fundamental structural unit is tropo-collagen, a molecular rod about 2600A in length and 15A in diameter and 300,000 molecular weight. In tendons these macromolecules, grouped as collagen fibrils, run parallel to the axis. In skin the fibrils are interlaced and branched. Applications: S:uitable for use as Type III collagen standard, Antigen for antibody production, Coating material for cell culture studies and Formation of collagen gels. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Controlled and limited pepsin digestion, followed by selective salt precipitation. > 90% as judged by SDS-PAGE analysis.
Form:
Supplied as a solution of collagen in 500mM acetic acid (C = 0.5mg/ml). The collagen may be transferred into physiological buffers by dialysis at 2-8C.
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