Applications: Suitable for use as a control or standard in indirect trapping ELISA, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 1:10,000 Western Blot: 1:1000 Immunochemistry: 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and freeze at -20C or colder. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purified by immunoaffinity chromatography. It is supplied free from other collagens, human serum proteins, and non-collagen extracellular matrix proteins
Form:
Supplied as a liquid in 0.5M acetic acid, 0.01% sodium azide. No stabilizing proteins added.
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