Recombinant protein corresponding to Glu19-Glu569 of human Complement Factor H-Related 5 expressed in NSO cells.
Complement factor Hrelated protein 5 (FHR5 or CFHR5) is a 65 kDa member of the factor Hrelated protein family. It is secreted by the liver and circulates either free or bound to HDL. It is unique among FHR proteins in that it associates with complexes that arise from complement activation. It promotes C3b cleavage and blocks C3 convertase activity. Human CFHR5 is 551 amino acids (aa) in length. It contains nine, 60 aa SUSHI, or short consensus repeats, plus 18 intrachain disulfide bonds. SUSHI repeats 57 (aa 267444) reportedly mediate CFHR5 binding to CRP and heparin. The complement interaction site(s) is unknown. Human CFHR5 shares 64% aa sequence identity with canine CFHR5.. Applications: Suitable for use in Western Blot, Immunoprecipitation and Direct ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml. C7850-88A under non-reducing conditions only. Immunoprecipitation: 25ug/ml. Conditioned cell culture medium spiked with C7850-88A Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
