Connexin 32 (CX32, CB1, Gap Junction Beta1 Protein, Gap Junction Liver Protein) (MaxLight 650), Clone: [2Q252 M12.13], Mouse, Monoclonal
Biozol Catalog Number:
USB-C7854-02-ML650
Supplier Catalog Number:
C7854-02-ML650
Alternative Catalog Number:
USB-C7854-02-ML650-100
Manufacturer:
US Biological
Host:
Mouse
Category:
Antikörper
Application:
IHC, WB
Immunogen:
Directed against an epitope located between residues 95-125 in the central cytoplasmic loop of rat Cx32.
MaxLight(TM)650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor(TM)647, DyLight(TM)649, Cy5(TM) and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (655nm), Emission (676nm), Extinction Coefficient 250,000. Applications: Suitable for use in Immunohistochemistry (lightly formaldehyde fixed tissues) and Western Blot. Other applications have not been tested. Recommended Dilution: Western blot: Detects a 27kD Connexin 32 polypeptide in blots of rat hepatocyte gap junctions and an additional 47kD band corresponding to aggregates. Optimal dilution to be determined by the researcher. Hybridoma: Produced by the immunization of BALB/c mice with isdolated rat junctional complexes. Storage and Stability: Store product at 4C in the dark. DO NOT FREEZE! Stable at 4C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight(TM)650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.
