Synthetic peptide derived from the C-terminal sequence of the mouse connexin 32 protein.
Intercellular communication mediated by gap junctions plays an important role in a variety of cellular processes, including homeostasis, morphogenesis, cell differentiation, and regulation of growth.1-3 Gap junctions are transmembrane channels that directly link neighboring cells by regulating the exchange of low molecular weight (<1200D) metabolites, ions, and second messengers. Gap junctions are formed by the interaction of hemichannels (connexons) on adjacent cells. Each connexon is composed of a hexameric assembly of connexin proteins. Connexins are highly homologous proteins encoded by a multigene family with similar structural features, including a cytoplasmic N-terminal region, four transmembrane domains, two extracellular loops, and a C-terminal cytoplasmic tail. Comparison of the amino acid sequence of the various connexin family members indicates that the two areas of greatest divergence are the intracellular loop connecting the second and third transmembrane domains and the C-terminal tail.1-2 These distinct domains are thought to mediate connexin type-specific properties, including phosphorylation, responses to gating stimuli, and assembly and membrane turnover. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1-5ug/mL Immunohistochemistry (4% paraformaldehyde): 0.5-2.0ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20C. Aliquots are stable at -20C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
Purity:
Purified by immunoaffinity chromatography.
Form:
Supplied as a liquid in PBS, pH 7.2. No preservatives added. Labeled with Fluorescein isothiocyanate (FITC).
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