Connexin 33 (CX33, Gap Junction Alpha 7 Protein, CXA-7), Rabbit
Biozol Catalog Number:
USB-C7854-27A
Supplier Catalog Number:
C7854-27A
Alternative Catalog Number:
USB-C7854-27A-100
Manufacturer:
US Biological
Host:
Rabbit
Category:
Antikörper
Application:
ELISA
Immunogen:
Synthetic prptide corresponding to 17aa near the C-terminal cytoplasmic domain of rat CX33
Gap junctions are composed of transmembrane channels that link the cytoplasm of neighboring cells. They differ from other membrane channels since they exist between two cells. Gap junctions are relatively non-specific and allow passive diffusion of small molecules up to 1000 Daltons. The junctions exist in almost all vertebrate and non-vertebrates cells. It is believed that gap junction play an important for intercellular communications and affect growth and differentiation of cells. Absence of intercellular communication mediated by gap junction may lead to transformed or cancerous growth. Gap junctional channel is composed of a hemichannel (connexon) in the cell membrane of one cell joined in mirror symmetry with a connexon in the opposing cell. Each connexon is an oligomer of six protein subunits that define the axial aqueous pore. Molecular cloning studies have identified a family of at least 12 highly related Connexins that are designated according to mol. wt, CX26-50. Hydropathy analyses of CX sequences predicts 4 transmembrane domains, 2 extracellular (EC), and 3 cytoplasmic (CP) domains. The EC, TM, and N-terminal CP domains are well conserved among family members, while Central and C-terminal domains are highly variable in both sequence and size. The N and C-termini are predicted to be cytoplasmic. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 1:100,000 using 50-100ng control peptide/well Optimal dilutions to be determined by the researcher. Controlo Peptide: C7854-27B: Connexin 33, Rat (CX33, Gap Junction Alpha 7 Protein, CXA-7) Control Peptide Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified by immunoaffinity chromatography
Form:
Supplied as a lyophilized powder from PBS, 0.1% BSA. Reconstitute with 100ul sterile PBS.
* VAT and and shipping costs not included. Errors and price changes excepted
