Copper Assay Kit, BioAssay(TM)

Product Overview

• Article Name: Copper Assay Kit, BioAssay(TM)
• Biozol Catalog Number: USB-C7900-59K
• Supplier Catalog Number: C7900-59K
• Alternative Catalog Number: USB-C7900-59K-250
• Manufacturer: US Biological
• Category: Kits/Assays

Product Description

Copper is an essential trace element. Copper-containing enzymes play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of hemoglobin, elastin and collagen. Copper is mainly present in caeruloplasmin in the liver. Low levels of copper have been associated with mental retardation, depigmentation, anaemia, hypotonia and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as Wilsons disease, microcytic hypochromic anaemia and bone disease due to reduced collagen synthesis. Simple, direct and automation-ready procedures for measuring copper concentrations find wide applications in research, drug discovery and environmental monitoring. Copper assay kit is designed to measure copper with no or minimal sample treatment. The improved method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the color, measured at 359nm, is directly proportional to copper concentration in the sample. The optimized formulation substantially reduces interference by substances in the raw samples. Key Features: Sensitive and accurate. Linear detection range 7 ug/dL (1.0uM) to 300ug/dL (47uM) copper in 96-well plate assay. Simple and high-throughput. The simple procedure can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day. Improved reagent stability and versatility. The optimized formulation has greatly enhanced reagent and signal stability. Cuvet or 96-well plate assay. Applications: Direct Assays: biological, environmental, food and beverage samples. Drug Discovery/Pharmacology: effects of drugs on Cu metabolism. Kit Contents (250 tests in 96-well plates): Reagent A: 10ml Reagent B: 1.5ml Reagent C: 40ml Copper Standard: 1ml 1.5 mg/dL Cu2+ Storage conditions. The kit is shipped at room temperature. Store all reagents at 4 C. Shelf life: 12 months after receipt. Precautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information. Procedures: Note: metal chelators (e.g. EDTA) interfere with this assay and should be avoided in sample preparation. Procedure using 96-well plate: 1. Standards: transfer 100ul dH2O into one Eppendorf tube labeled ''Blank. Into another tube labeled ''Standard, mix 20ul 1.5 mg/dL Standard and 80ul dH2O (final 300 ug/dL Cu2+). Samples: transfer 100ul samples into separate tubes. Add 35ul Reagent A (trichloroacetic acid) to each tube and mix by vortexing. If samples contain protein (e.g. serum/plasma), precipitates form. Centrifuge tubes for 2 min at 14,000 rpm and use clear supernatant for assay. For samples that do not contain protein, the mixture remains clear and centrifugation is not necessary. Transfer 100ul Blank, Standard and Sample into separate wells of a clear flat-bottom 96-well plate. 2. For each assay well, prepare Working Reagent by mixing 5ul Reagent B and 150ul Reagent C. Transfer 150ul Working Reagent to each well and tap plate to mix thoroughly. 3. Incubate 5 min at room temperature and read optical density at 356-362nm (peak absorbance at 359nm). Note: if sample OD values are higher than the OD value for the 300ug/dL Standard, dilute sample in dH2O and repeat assay. Multiply the results by the dilution factor. Procedure using cuvette: Prepare standards and samples as for 96-Well Assay Procedure::. 1. Transfer 400ul Standards and Samples into separate cuvets. 2. Add 600ul Working Reagent. Mix by pipetting. 3. Incubate 5 min at room temperature and read optical density at 356-362nm (peak absorbance at 359nm). Calculation: The copper concentration of Sample is calculated as = ODSAMPLE-ODBLANK ODSTANDARD-ODBLANK * 300 (ug/dL) ODSAMPLE, ODBLANK and ODSTANDARD are optical density values of the Sample, Blank and the 300 ug/dL Standard, respectively. Conversions: 100 ug/dL Cu equals 15.5uM, 0.0001% or 1