CRACC, Recombinant, Mouse (SLAM Family Member 7, CD2-like Receptor-Activating Cytotoxic Cells, SLAMF7)
Biozol Catalog Number:
USB-C7906-72
Supplier Catalog Number:
C7906-72
Alternative Catalog Number:
USB-C7906-72-50
Manufacturer:
US Biological
Category:
Molekularbiologie
CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a 66kD type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature mouse CRACC consists of a 202 amino acid (aa) extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain, a 21 aa transmembrane segment, and an 88 aa cytoplasmic domain with two immunoreceptor tyrosine-based switch motifs ITSMs (2, 3). Within the ECD, mouse CRACC shares 53% aa sequence identity with human CRACC. It shares 19%-35% aa sequence identity with comparable regions of other mouse SLAM proteins including 2B4, BLAME, CD2F-10, CD84, CD229, NTB-A, and SLAM/CD150. Additional isoforms of mouse CRACC are distinguished by deletions and/or substitutions in their cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8+ T cells, activated B cells, and mature dendritic cells (4, 5). It interacts homophilically to induce NK, CTL, and B cell activation (4-7). In human NK cells, activated CRACC transmits signals following association with the adaptor protein EAT-2 (8). A DNA sequence encoding the extracellular domain of mouse CRACC (Ala 22-Gly 224, Accession Q8BHK6) was fused to a 10X histidine tag at the C-terminus. The protein was expressed in Chinese Hamster Ovary cells. Calculated molecular mass of 23.6kD. As a result of glycosylation, the recombinant protein migrates as an ~ 34-55kD protein in SDS-PAGE under reducing conditions. Activity: Measured by its ability to bind biotinylated rmCRACC. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
