C-Reactive Protein, Rat (CRP, MGC88244, PTX 1, PTX1)
Biozol Catalog Number:
USB-C7907-16A
Supplier Catalog Number:
C7907-16A
Alternative Catalog Number:
USB-C7907-16A-10
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
WB
Pentraxins family of proteins acquired the name from their ability to form pentameric (or decameric) structures formed by non-covalent interactions. C-reactive protein (CRP or PTX1, mature chain 206 aa, chromosome 1q21-23) nonglycosylated, ~24kD monomer and ~118kD pentamer) is a ubiquitous protein found in both vertebrates and invertebrates. Originally CRP was defined as a substance, observed in the plasma of patients with acute infections, that reacted with the C polysaccharide of the pneumococcus. It is one of the plasma proteins that are called acute phase reactants because of a pronounced rise in concentration after tissue injury or inflammation, in the case of CRP the rise may be 1000-fold or more. CRP is composed of 5 identical, 21,500-molecular weight subunits. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver, those cells produce CRP detectable on lymphocytes. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 50-500ng/lane ELISA: 1ug/ml for coating or use as a standard. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified ~90% by SDS-PAGE, RID and IEP.
Form:
Supplied as a lyophilized powder from Tris, pH 7.5, 0.2M NaCl, 2mM CaCl2, 0.05% sodium azide.
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