Creatine Kinase MB Isoenzyme II, Recombinant (CKMB) (Pichia)
Biozol Catalog Number:
USB-C7910-01J
Supplier Catalog Number:
C7910-01J
Alternative Catalog Number:
USB-C7910-01J-1
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Recombinant full length Creatine Kinase MB Isoenzyme with aa sequence identical to native enzyme. CK-MB is a dimeric protein comprised of M and B subunits. The doublet band migrating approximately 47kD is not resolved on most gels. Purified in the enzymatically active form. Reacts with monoclonal antibodies specific to MB isoenzyme in ELISA. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Unit Definition: One unit equals 1 micromole creatine phosphate formed/minute. Enzyme Activity: 700u/mg at 37C at 340nm Storage and Stability: Aliquot to avoid repeated freezing and thawing. Store at -70C. Aliquots are stable for 6 months after receipt at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Form:
Supplied as a liquid in 10mM Tris-HCl, pH 6.8, 0.5mM DTT, 0.5mM EDTA, 50% glycerol. No preservative added.
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