Creatine Kinase MM Isoenzyme I, Recombinant (CKMM) (Pichia)
Biozol Catalog Number:
USB-C7910-27V
Supplier Catalog Number:
C7910-27V
Alternative Catalog Number:
USB-C7910-27V-1
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA, WB
Recombinant full length Creatine Kinase MM isoenzyme without the C-terminal lysine on both subunits. CK-MM is a 47kD dimeric protein comprised of 2 identical subunits of Type 1 sequence. Purified in the enzymatically active form. Reacts with polyclonal antibodies specific to MM isoenzyme in ELISA. Applications: Suitable for use in ELISA and Western Blot. Major band migrating above 37kD in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Unit Definition: One unit will transfer one micromole of phosphate from creatine phosphate to ADP per minute at 37C. Measured at 340nm as one equimolar amount of NADH produced by coupled reaction. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Purity:
~95% (SDS-PAGE). Purified under non-denaturing conditions.
Form:
Supplied as a liquid in 0.01M Tris-HCl, pH 7.0-7.5, 0.075M sodium chloride, 10mM beta-mercaptoethanol, 0.09% sodium azide, 50% glycerol.
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