Creatine Kinase MM Isoenzyme III, Recombinant (CKMM) (Pichia)
Biozol Catalog Number:
USB-C7910-28C
Supplier Catalog Number:
C7910-28C
Alternative Catalog Number:
USB-C7910-28C-1
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA
Creatine Kinase MM isoenzyme with identical amino acid sequence to that of the native enzyme. CK-MM is a 47kD dimeric protein comprised of 2 identical subunits. Purified in the enzymatically active form. Reacts with polyclonal antibodies to MM isoenzyme in ELISA. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Unit Definition: One unit will transfer one micromole of phosphate from creatine phosphate to ADP per minute at 37C. Measured at 340nm as one equimolar amount of NADH produced by coupled reaction. Storage and Stability: May be stored at 4C for short-term only. For long-term storage, aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.
Purity:
~95% (SDS-PAGE). Purified under non-denaturing conditions.
Form:
Supplied as a liquid in TBS, 10mM beta-mercaptoethanol, pH 7.0-7.5, 0.09% sodium azide, 50% glycerol.
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