CREB, phosphorylated (Ser133) (cAMP Response Element Binding Protein) Blocking Peptide
Biozol Catalog Number:
USB-C7915-10A
Supplier Catalog Number:
C7915-10A
Alternative Catalog Number:
USB-C7915-10A-100
Manufacturer:
US Biological
Category:
Molekularbiologie
Application:
ELISA, WB
Transcription factor CREB binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth or neurotrophic factors. Protein kinase A and the calmodulin-dependent protein kinase CaMKII stimulate CREB phosphorylation at Ser133, a key regulatory site controlling transcriptional activity (1,2). Phosphorylation at Ser133 is also mediated by p44/42 MAP kinase, p90RSK, p38 MAP kinase and MSK1 (3-5). CREB appears to play an important role in learning and memory in both flies and mice. Produced by immunizing rabbits with a synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser133 of human CREB. Corresponding antibody, C7915-10, detects endogenous levels of CREB only when phosphorylated at serine 133. Also detects the phosphorylated form of CREB-related protein ATF-1. Species Crossreactivity: Mouse and rat. Applications: Suitable for use in ELISA, Western Blot or for antigen applications in immunological protocols. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C or colder. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Purity:
Purified
Form:
Supplied as a lyophilized powder.
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