A 21aa peptide sequence within the C-terminus of mouse CRY1. 85% conserved in monkey, human, 76% in chicken and 71% in frog CRY1. No significant homology is seen with CRY2. Control peptide, because of its low MW (<3kD), is not suitable for Western Blot. Circadian rhythm is one of the most fascinating and complex biological phenomenons. The circadian clock controls biological activities on daily light-dark cycles in species from cyanobacteria to humans. The circadian clock has three major components: A photoactive pigment (chromophore) for sensing light and transmitting light signals, the circadian clock that oscillates every ~24-hrs, and the genes controlled by the circadian clock to bring about the physiological and behavioral changes. Several genes (Drosophila Clock Per, Tim, mammalian Per1, Per2, Per3, MOP3 and MOP4, and BMAL1) have been linked to rhythmicity or circadian behavior of living organisms. However, very little is known about the mammalian photosensory molecules. Most recently mammalian homolog of the plant blue-light photoreceptors termed cryptochromes have been identified. Mouse CRY1 and CRY2, are 606 aa and 569 aa protein, respectively. Cry1 and Cry are specifically expressed in ganglion cell and inner nuclear layers of the mouse retina. CRY1 is expressed at high level in the SCN and oscillates in a circadian manner. Like other genes implicated in circadian mechanism, CRY1 and CRY2 are also expressed in most animal tissues. Therefore, mammalian cryptochromes appears to play an important role in entrainment of the circadian clock. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 50-100ng Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Purity:
Highly purified
Form:
Supplied as a liquid in PBS, pH 7.5
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