CTDSP1, Recombinant, Human, aa1-260, His-Tag (Carboxy-terminal Domain RNA Polymerase II Polypeptide A Small Phosphatase 1, Nuclear LIM Interactor-interacting Factor 3, NLI-interacting Factor 3, NLI-IF, NIF3, NLIIF)
Biozol Catalog Number:
USB-C7947-98F
Supplier Catalog Number:
C7947-98F
Alternative Catalog Number:
USB-C7947-98F-50,USB-C7947-98F-250
Manufacturer:
US Biological
Category:
Molekularbiologie
Carboxy-terminal domain, RNA polymerase II, polypeptide A phosphatase 1, also known as CTDSP1, is a class 2C phosphatase with activity dependent on the conserved DxD motif. CTDSP1 preferentially catalyzes the dephosphorylation of Ser-5 within the tandem 7 residues repeats in the C-terminal domain (CTD) of the largest RNA polymerase II subunit POLR2A. Also it negatively regulates RNA polymerase II transcription, possibly by controlling the transition from initiation/capping to processive transcript elongation. Source: Recombinant corresponding to aa1-260 from human CTDSP1, fused to His-tag at N-terminus expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MDSSAVITQI SKEEARGPLR GKGDQKSAAS QKPRSRGILH SLFCCVCRDD GEALPAHSGA PLLVEENGAI PKTPVQYLLP EAKAQDSDKI CVVIDLDETL HSSFKPVNN ADFIIPVEID GVVHQVYVLK RPHVDEFLQR MGELFECVLF TASLAKYADP VADLLDKWGA FRARLFRESC VFHRGNYVKD LSRLGRDLRR VLILDNSPAS YVFHPDNAVP VASWFDNMSD TELHDLLPFF EQLSRVDDVY SVLRQPRPGS Molecular Weight: ~31.2kD (280aa), confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing.. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
