Cytokeratin 8 (Cytokeratin-8, CK 8, CK8, CK-8, Cyk8, Keratin 8, Keratin-8, K8, KRT8, Keratin Type II, Cytoskeletal 8, K2C8, Keratin, Type II Cytoskeletal 8), Rabbit

Product Overview

• Article Name: Cytokeratin 8 (Cytokeratin-8, CK 8, CK8, CK-8, Cyk8, Keratin 8, Keratin-8, K8, KRT8, Keratin Type II, Cytoskeletal 8, K2C8, Keratin, Type II Cytoskeletal 8), Rabbit
• Biozol Catalog Number: USB-C9097-42A
• Supplier Catalog Number: C9097-42A
• Alternative Catalog Number: USB-C9097-42A-100
• Manufacturer: US Biological
• Host: Rabbit
• Category: Antikörper
• Application: IHC, WB
• Immunogen: Synthetic peptide corresponding to a sequence at the N-terminal of human CK8, different to the related rat sequence by a single amino acid.

Product Description

Cytokeratin 8 (CK8), also known as Keratin 8 (K8), is a type II keratin. Among the various intermediate filament (IF) proteins, cytokeratin 8 (CK8) is especially remarkable as it is produced early in embryogenesis, is the only type-II CK occurring in many simple epithelial cells, and can also be synthesized in certain non-epithelial cells. Cytokeratin 8 mRNA was abundant in fetal small intestine, placenta, pancreas, lung, liver, and kidney. Levels of cytokeratin 8 mRNA in placenta increased slightly during pregnancy. He et al. (2002) presented evidence that K8 is a new cytoplasmic target for JNK in Fas receptor-mediated signaling. The functional significance of this phosphorylation could relate to regulation of JNK signaling and/or regulation of keratin dynamics. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot:1ug/ml Immunohistochemistry (paraffin):2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Purity: Purified by immunoaffinity chromatography.
• Form: Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.