Dimethylarginine dimethylaminohydrolase 1, also known DDAH1, belongs to the dimethylarginine dimethylaminohydrolase gene family. The DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Impairment of DDAH1 causes ADMA (asymmetric dimethylarginine) accumulation and a reduction in cGMP generation. Source: Recombinant corresponding to aa1-285 of human DDAH1 protein, fused to His-tag at N-terminus, expressed in E.coli. Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMAGLGHP AAFGRATHAV VRALPESLGQ HALRSAKGEE VDVARAERQH QLYVGVLGSK LGLQVVELPA DESLPDCVFV EDVAVVCEET ALITRPGAPS RRKEVDMMKE ALEKLQLNIV EMKDENATLD GGDVLFTGRE FFVGLSKRTN QRGAEILADT FKDYAVSTVP VADGLHLKSF CSMAGPNLIA IGSSESAQKA LKIMQQMSDH RYDKLTVPDD IAANCIYLNI PNKGHVLLHR TPEEYPESAK VYEKLKDHML IPVSMSELEK VDGLLTCCSV LINKKVDS Molecular Weight: 33.5kD (308aa) confirmed by MALDI-TOF Enzyme Activity: Not determined. This product is recommended for use in applications that do not require a catalytically active form of the protein. Storage and Stability: May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
