Desmin, Recombinant, Human (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)

Product Overview

• Article Name: Desmin, Recombinant, Human (DES, CMD1I, CSM1, CSM2, FLJ12025, FLJ39719, FLJ41013, FLJ41793, Intermediate Filament Protein)
• Biozol Catalog Number: USB-D3221-14
• Supplier Catalog Number: D3221-14
• Alternative Catalog Number: USB-D3221-14-100
• Manufacturer: US Biological
• Category: Molekularbiologie
• Application: ELISA, WB

Product Description

Desmin is the main intermediate filament in mature skeletal, cardiac and smooth-muscle cells. Can be used to distinguish rhabdomyosarcomas and leiomyosarcomas from other soft tissue tumors, lymphomas and carcinomas. Molecular Weight: Calculated from sequence: 53.5396kD Observed (SDS Gel Electrophoresis): 55kD Isoelectric Point: pI 5.16 (calculated from sequence). Applications: Suitable for use as protein standard in 1D and 2D SDS gel electrophoresis, in Immunoassays and in immunization. Other applications not tested. Reconstitution to Filaments: Protofilaments and filament complexes are obtained by dialyzing the reconstituted polypeptide solution stepwise to a concentration of 4M urea and then to low salt condition (50mM sodium chloride, 2mM DTT, 10mM Tris-HCl, pH 7.4) For Use in Immunization: The solution can be further dialyzed against PBS (phosphate buffered saline, e.g. Dulbeccos PBS). Storage and Stability Store lyophilized product at -20C. Store reconstituted product in aliquots at -20C to avoid repeated freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Properties

• Molecular Weight: 53.5396
• NCBI: 17661
• UniProt: P17661
• Purity: 95% (determined by SDS gel electrophoresis).
• Form: Supplied as a lyophilized powder. Reconstitute with 70ul sterile water. Final Solution contains: 30mM Tris-HCI, pH 8, 9.5M urea, 2mM DTT, 2mM EDTA, 10mM methylammonium chloride.