Desmoglein 3, Fc Chimera, Recombinant, Human (BSA Free)
Biozol Catalog Number:
USB-D3221-84C
Supplier Catalog Number:
D3221-84C
Alternative Catalog Number:
USB-D3221-84C-50
Manufacturer:
US Biological
Category:
Molekularbiologie
Desmoglein-3, also known as pemphigus vulgaris antigen (PVA), is a 130kD transmembrane glycoprotein that belongs to the cadherin family of calcium dependent adhesion molecules (1-3). Human Desmoglein-3 is synthesized with a 26aa propeptide. The mature protein consists of a 566aa extracellular domain (ECD) that contains four cadherin-like domains, a 25 aa transmembrane segment, and a 359aa cytoplasmic domain (4). Within the ECD, human Desmoglein-3 shares 78% aa sequence identity with mouse and rat Desmoglein-3. It shares 44%, 47%, and 56% aa sequence identity with Desmoglein-1, -2, and -4 respectively. Desmoglein-3 is one of the principal components of desmosomes which form adhesive contacts between epithelial cells (1, 2). It is expressed in the basal and suprabasal layers of stratified epithelia in many tissues (4-6). During apoptosis, Desmoglein-3 is cleaved by caspases, plus MMP-2, and MMP-9 at sites within the cytoplasmic and extracellular regions, resulting in shortened transmembrane forms and a soluble 75kD ECD fragment (7, 8). The downregulation of Desmoglein-3 in oral squamous cell carcinoma correlates with metastatic potential (9). Desmoglein-3 is the target of autoantibodies in pemphigus vulgaris, a blistering skin disorder with compromised inter-keratinocyte adhesion (6, 10). Binding of these antibodies triggers Desmoglein-3 internalization and degradation by keratinocytes (10). A DNA sequence encoding the extracellular domain of human Desmoglein-3 (Met 1-Arg 615, Accession P32926) was fused to the Fc region of human IgG1 via a polypeptide linker. The chimeric protein was expressed in a mouse myeloma cell line, NS0. Calculated molecular mass of 89.5kD. As a result of glycosylation, the recombinant protein migrates as an ~ 105-110kD protein in SDS-PAGE under reducing conditions. Activity: Measured by the ability of the immobilized protein to support the adhesion of Caki-2 (human renal carcinoma epithelial cell line) cells. Storage and Stability: Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
